Composite

Part:BBa_K1937010

Designed by: Oumnia KARIM   Group: iGEM16_Toulouse_France   (2016-10-14)


Epsilon/MazF toxin and antitoxin in pSBBS0K-mini

Chassis B. subtilis, carrier plasmid pSBBs0K-mini)
Length: 6855 bp
Background:
This Epsilon/MazF part is one of the two components of a double toxin-antitoxin system. This part contains Epsilon/MazF (BBa_K1937009) carried on the plasmid pSBBs0K-mini (BBa_K1937001). This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

BBa K193709-description.png

BBa K1937010-map.png Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 257
    Illegal suffix found in sequence at 285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 257
    Illegal NheI site found at 279
    Illegal SpeI site found at 286
    Illegal PstI site found at 300
    Illegal NotI site found at 263
    Illegal NotI site found at 293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 257
    Illegal BglII site found at 4814
    Illegal BamHI site found at 330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 257
    Illegal suffix found in sequence at 286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 257
    Illegal XbaI site found at 272
    Illegal SpeI site found at 286
    Illegal PstI site found at 300
    Illegal NgoMIV site found at 1877
    Illegal AgeI site found at 4425
    Illegal AgeI site found at 5387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 1701
    Illegal BsaI.rc site found at 3140
    Illegal BsaI.rc site found at 5656
    Illegal SapI site found at 618
    Illegal SapI.rc site found at 4638


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
biology